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3. Optical metrology and microscopy Because a hologram can produce an incredibly accurate replica of a wavefront recorded at another place at another time, the images it produces can be measured with great precision. A room-sized nuclear containment vessel can be recorded in a laser flash, for example, and its image then examined at leisure at a distant and nonhazardous laboratory for cracked metal parts, corrosion, and so forth. The same property can also be used for very small subjects. In ordinary photography, the higher the resolution that is needed, the shallower the depth of field that can be focused. Thus a normal microscope can t be used to make direct measurements on moving three-dimensional arrangements of small things, but a holography enables capturing a snapshot of the entire volume which can then be examined later. The nuclear physics team at MIT built a holographic recording system for a giant-size bubble chamber (3 meters deep) used in the search for the tau lepton. Holography allows 30 micron bubbles to be tracked throughout the depth of the chamber.

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If you add an anchor point to a straight segment (one that has no control handles on either end), the anchor point becomes a straight corner point If the segment is curved meaning that you have at least one control handle for that segment the new anchor point becomes a smooth point Delete Anchor Point tool: This deletes the anchor point you clicked Illustrator creates a new segment between the anchor points that were on either side of the anchor point you clicked If the anchor point on which you clicked is an endpoint, no new segment is drawn; instead, the next or previous anchor point on the path becomes the new endpoint Scissors tool: You use this tool to split paths Clicking with the Scissors tool on a closed path makes that path an open path, with the endpoints directly overlapping each other where the click occurred.

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at pH 6 and below, in a process mediated via a putative transitional state (intermediate state) (see Figures 48 and 413 4) The close correlation between binding af nity and conformational data suggested the important conclusion that pH-driven trans-conformational changes are the reason for the changes in binding af nity with pH (conformational pH switch mechanism), implying that only the alkali state of Hsp47 is competent to bind procollagenlike model peptide (PPG)10 and by implication procollagen/collagen Importantly, the results of all these titration experiments and the conformational pH switch mechanism actually appear to make sense biologically Hsp47 is known to bind to and assist procollagen assembly in the endoplasmic reticulum (neutral mildly alkali pH) (a major site of protein synthesis is eukaryotic cells) and then remain bound to assist protein traf cking to the cis-Golgi (approximately pH 6.

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4) (a major site of protein storage before export from eukaryotic cells), where procollagen molecules then dissociate from Hsp47 to begin the process of brilisation prior to export from cells Hsp47 is then apparently returned to the endoplasmic reticulum in order to repeat the process Since the conformational pH-switch mechanism is central to the biological activity of Hsp47, further understanding is essential Detailed site-directed mutagenesis (protein engineering) studies are a powerful way forward (see 3) In the case of Hsp47, histidine amino-acid residues found in histidine clusters (breach, shutter and gate regions) were systematically mutated in turn to alanine residues Results demonstrated that breach and gate histidines appeared to control and modulate the conformational pH switch, with H191 acting as a potential trigger residue to initiate the correct pH-driven conformational change process (Figure 739).

Using the Scissors tool on an open path splits that open path into two separate open paths, each with an endpoint that overlaps the other open path s endpoint Knife tool: This tool slices through path areas It s the only path-editing tool that doesn t require you to have paths selected; it works on all unlocked paths that fall under the blade Use this to cut an object into two closed-path objects Convert Direction Point tool: This tool has two functions The first is to simply change an anchor point from its current type to a straight corner point by clicking and releasing it You can also change the current type to Smooth by clicking and dragging on the anchor point.

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